Automated Serial Dilution with the Genie GLS1
Overview
Serial Dilution is a series of dilutions where the concentration decreases by the same factor in each step. Serial dilutions are used across many scientific applications, and automating this process saves labs time, effort, and human error. The Genie Serial Dilution protocol requires users to input the number of dilutions, dilution factor, and total well volume. Using these inputs, Genie calculates how much stock liquid and diluent to transfer to your serial dilution wells. The Serial Dilution transfers the stock solution and diluent from a 4 column reservoir and completes each dilution in a new column of a 96 flat-bottom plate, with a single well liquid waste reservoir.
Serial Dilution is useful in many applications such as:
- Microbiology
- Biochemistry
- Molecular Biology
- Proteomics
- Immunology
- Forensics
- and more…
Workflow
1
Open the Serial Dilution protocol and run the protocol in simulation.
2
Create your protocol's instrument settings.
3
Input the number of dilutions, dilution factor, and total volume.
4
Set up your instrument's deck, and run your protocol.
Materials
- (1) 4-column reservoir
- (1) 96 well assay plate, flat-bottom, 200uL
- (1) Single well reservoir, 240mL
- (1-2) Tip Boxes (number and tip sizes dependent on user’s runtime specifications)
