Automated Pooling with the Genie GLS1
Overview
Sample pooling involves mixing multiple samples together in one batch, to be subsequently tested as one combined sample. Sample pooling increases testing resource efficiency, while automating this process saves labs time and reagents. The Genie Pooling protocol pools PCR plate samples into 2mL tubes. At runtime, users are asked to input sample volume to be pooled and a .csv file that specifies each sample’s origin plate, plate position, and destination tube.
Workflow
1
Open the Pooling protocol and run the protocol in simulation.
2
Create your protocol's instrument settings.
3
Create the sample .csv file. Create three .csv headers: Sample Position, Sample Plate ID, and Destination Tube. Input each column's information for each sample in a row. The number of rows you input equals the number of samples.
For example:
Row = 1, Sample Position = A1, Sample Plate ID = Sample Plate 1, Destination Tube = A1.
4
Input the sample pooling volume and the .csv file.
5
Set up your instrument's deck, and run your protocol.
Materials
- (1-3) 96 well PCR Plate, full skirted,150uL
- (1) 4×6 24-Tube Rack with (1) 2mL tube
- (1) 50uL Tip Box
