Automated PCR Sample Preparation with the Genie GLS1
Overview
PCR (polymerase chain reaction) is a common process for molecular biology. It is used in applications such as cloning, gene expression analysis, genotyping, sequencing, and more. Automating the PCR sample preparation can save labs time, effort, and accuracy. The Genie PCR Sample Preparation protocol transfers prepared PCR Master Mix and samples to PCR reaction wells. At runtime, users input the samples they would like to prepare for PCR, their respective volumes, and the total reaction volume. Genie will calculate the volume of master mix to transfer to the samples.
Workflow
1
Open the PCR Sample Preparation protocol and run the protocol in simulation.
2
Create your protocol's instrument settings.
3
Select the wells on the sample plate to prepare for PCR, and input the PCR reaction volume per well, and sample volume to transfer.
4
Set up your instrument's deck, and run your protocol.
Materials
- (1) 12-column reservoir
- (1) 96 well assay plate, flat-bottom, 200uL
- (1) 96 well PCR plate, full skirted, 200uL
- (1-2) Tip Boxes (number and tip sizes dependent on user’s runtime specifications)
