Automated PCR Master Mix Preparation with the Genie GLS1
Overview
PCR (polymerase chain reaction) is a common process for molecular biology. It is used in applications such as cloning, gene expression analysis, genotyping, sequencing, and more. Automating the preparation of master mix reagents can save labs time, effort, and accuracy. The Genie PCR Master Mix Preparation protocol combines PCR Master Mix Buffer, Water, Forward Primer, and Reverse Primer. At runtime, users input the number of PCR reactions to prepare master mix for and the volumes of each reagent per reaction.
Workflow
1
Open the PCR Master Mix Preparation protocol and run the protocol in simulation.
2
Create your protocol's instrument settings.
3
Input the number of reactions, PCR Master Mix volume per reaction, water volume per reaction, forward primer volume per reaction, and reverse primer volume per reaction.
4
Set up your instrument's deck, and run your protocol.
Materials
- (1) 12-column reservoir
- (1) 96-well midi plate, u-bottom, 0.8mL
- Tip Box(es) (number and tip sizes dependent on user’s runtime specifications)
