Pooling
Automated Pooling with the Genie LabMate
Overview
Sample pooling involves mixing multiple samples together in one batch, to be subsequently tested as one combined sample. Sample pooling increases testing resource efficiency, while automating this process saves labs time and reagents. The Genie Pooling protocol pools PCR plate samples into 2mL tubes. At runtime, users are asked to input sample volume to be pooled and a .csv file that specifies each sample’s origin plate, plate position, and destination tube.
Workflow
Step One
Open the Pooling protocol and run the protocol in simulation.
Step Two
Step Three
Create the sample .csv file. Create three .csv headers: Sample Position, Sample Plate ID, and Destination Tube. Input each column’s information for each sample in a row. The number of rows you input equals the number of samples. For example: Row = 1, Sample Position = A1, Sample Plate ID = Sample Plate 1, Destination Tube = A1.
Step Four
Input the sample pooling volume and the .csv file.
Step Five
Materials
- (1-3) 96 well PCR Plate, full skirted,150uL
- (1) 4×6 24-Tube Rack with (1) 2mL tube
- (1) 50uL Tip Box
