Normalization
Automated Normalization with the Genie LabMate
Overview
Normalization is the process of diluting samples with variable concentrations to a uniform concentration. Normalization is a key step in many genomic and proteomic workflows. Automating normalization can save labs time, effort, and accuracy. The Genie Normalization protocol normalizes samples from a 96 flat bottom assay plate and diluent from a 3-column reservoir to a 96 well PCR normalization plate. At runtime, users input the concentration and volume to normalize (up to 8 samples) as well as the initial concentration of each sample.
Workflow
01
Step One
Open the Normalization protocol and run the protocol in simulation.
02
Step Two
Create your protocol’s instrument settings.
03
Step Three
Input your normalization volume, concentration, and sample (1-8) concentrations.
04
Step Four
Set up your instrument’s deck, and run your protocol.
Materials
- (1) 3-column reservoir
- (1) 96 well PCR Plate, 200uL
- (1) 96 well assay plate, flat-bottom, 392uL
- (1) Tip Box (tip sizes dependent on user’s runtime specifications)
